UVP GelDoc-It TS Imaging System User Manual

The GelDoc-It TS System is designed as a self-contained imaging system with a built-in computer, no external computer required. The system enables capture and documentation of fluorescent gels with the ability to save images to the internal system or to an external USB storage device for later quantitative analysis or enhancement for publication.

System Components Components Refer to the packing slip and pictured components for parts included with the system.

Operating System: Windows XP Processor: 1.5GHz (minimum) USB storage device capacity: 1G (minimum) Internal storage (hard disk): 60GB (minimum) USB 2.0 ports: 1 front, 4 in the back Software Options: TS software (supplied only with the manual lens)

VisionWorks®LS software (supplied with the motorized lens)

GelDoc-It TS Imaging System 3

GelCam 310 Camera, CCD monochrome model with a resolution of 1600 x 1200 with a USB 2.0 PC interface. All camera settings are factory pre-set for optimum performance when viewing gels, films, or membranes under low light level conditions. UVP Technical Support should be contacted before making any adjustments to the camera settings.

Lens Kit (Motorized or Manual) The zoom lens comes in two configurations.

 The motorized version is controlled via the computer.  The manual version needs manual adjustment from the user.

Both lenses are fitted with a close-up diopter. The diopter is used for focusing on objects at the focus length of the GelDoc-It TS System.

Ethidium Bromide Filter (EtBr)

The Ethidium Bromide (EtBr) Filter (50mm sq.) UV blocking band pass interference filter blocks UV and IR radiation emitted from the transilluminator. The filter is placed in the filter tray below the camera assembly. The filter allows visualization of fluorophores from 580-630nm, targeting the EtBr emission which peaks at 605nm. The EtBr filter can be substituted for other specific fluorophore filters or removed when imaging non-fluorescent media (protein gels, colony plates, etc.) in order to produce brighter images.

The darkroom is light tight for optimum imaging conditions. The darkroom features:

 Overhead white light  Roll out tray for the transilluminator  Gel viewer window  Side pocket for storage of materials  Brackets for installing optional UV lamps for overhead UV illumination  Built in power cord for an optional LED white light tray

Transilluminator

A transilluminator may be included with the system; refer to the packing list. The GelDoc-It TS System can accommodate UVP’s Benchtop and FirstLight transilluminator models. UVP offers a variety of configurations, from the benchtop models with 8-watt, single wavelength and single intensity to multiple wavelengths and high/low intensities, or the high-end FirstLight transilluminator.

Note: For UV protection, there is a 10-minute timer installed in the system. The timer shuts off the transilluminator after 10 minutes of operation. A switch on the front of the darkroom allows the user to reset the timer. When the darkroom door is opened, this automatically resets the timer.

LCD Touch Screen

The display is a 15-inch VGA color touch screen connected to the darkroom cabinet with tilt adjustment. The touch screen allows the user to preview, snap, save and print images, as well as select certain preference options, without the need of an external mouse or keyboard.

A stylus pen is included for increased pointer control in selecting software options on the touch screen.

Focus Target

The focus target fluoresces when placed on a transilluminator or when exposed to overhead UV. The target provides sharp, fluorescent images to aid in adjusting the focus of the camera.